[Colorimetric method for the quantitative determination of plasma catalase activity].
نویسندگان
چکیده
HE STUDY OF CATALASE ACTIVITY has been the subject of much investigation and has necessitated the use of very elegant technics. Recently, methods have been introduced which simplified the technics for estimating catalase activity. Among these are the iodimetric titration procedure of Dille and Watkins (1), the use of sodium perborate as a source of hydrogen peroxide (2), and various methods utilizing permanganate titration of undecomposed substrate (3, 4, 5). The purpose of this paper is to present a colorimetric method for the estimation of catalase activity which yields accurate, reproducible results, and is simplified so that clinical chemistry laboratories may perform this determination without the need for special equipment.
منابع مشابه
New Enzymatic Colorimetric Method for the Quantitative Determination of Phenylalanine in Dry-Blood Spots
Phenylketonuria (PKU) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. The symptoms of this disease can be prevented if detected soon after birth. Therefore, blood Phenylalanine (Phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of PKU patients. The goal of this research was to introduce a r...
متن کاملEvaluation of Oxidative Stress in Blood During Hemodialysis
The number of patients with end-stage renal disease is growing rapidly in Asia. Patients with end stage renal disease failure (ESRD) undergoing long term hemodialysis (HD) are at risk for high oxidative stress. There is paucity of information with regard to oxidative stress during HD. Therefore, the aim of the present study was to evaluate catalase enzyme activity in erythrocytes and the total ...
متن کاملPrecise Method for the Assessment of Catalase-like Activity in Seminal Fluids
In the present study, a sensitive modification of the colorimetric assay for catalase activity is reported. This assay is based on the reaction of undecomposed hydrogen peroxide with ammonium molybdate to give a yellowish color, which has maximum absorbance at 354 nm. Catalase-like activity is calculated from a comparison with the activity of a certified standard enzyme with known unit activity...
متن کاملDevelopment and Validation of a HPLC Method for Determination of Pefloxacin in Tablet and Human Plasma
Objective(s) Developing and validating a simple, efficient, reproducible and economic reversed phase high performance liquid chromatographic (RP-HPLC) method for the quantitative determination of pefloxacin in bulk material, tablets and in human plasma. Materials and Methods A shim-pack CLC-ODS column and a mobile phase constituting acetonitrile: 0.025 M phosphoric acid solution (13:87 v/v, ...
متن کاملChitosan Capped Silver Nanoparticles as Colorimetric Sensor for the Determination of Iron(III)
A selective, simple and low-cost method for the colorimetric determination of Fe3+ ions based on chitosan capped silver nanoparticles (Chit-AgNPs) was presented. Chitosan is a cationic polyelectrolyte and possesses amino and hydroxy groups which make it widely used as a capping agent for Ag NPs. The synthesized chitosan capped silver nanoparticles with excellent colloidal stability were charact...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 4 4 شماره
صفحات -
تاریخ انتشار 1958